An upstream activation sequence controls the expression of AOX1 gene in Pichia pastoris
β Scribed by Yaoji Xuan; Xiangshan Zhou; Wenwen Zhang; Xiao Zhang; Zhiwei Song; Yuanxing Zhang
- Book ID
- 109295644
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 533 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1567-1356
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The Pichia pastoris system has the potential for very high level production of foreign proteins. This, together with the recent availability of this system in kit form, has changed Pichia from being an expression system with only specialized biotechnological applications to one that is widely used f
The upstream region of the Saccharomyces cerevisiae pyruvate decarboxylase structural gene, PDC1, has been isolated and fused to the indicator gene Escherichia coli lacZ. 1.2 kb of the upstream region has been sequenced. The PDC1-lacZ fusion has been integrated at the ura3-52 locus in the yeast geno