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An ultramicro method for the measurement of pepsin

โœ Scribed by Michael D. Turner; Jacquelyn L. Tuxill; Leon L. Miller; Harry L. Segal


Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
716 KB
Volume
16
Category
Article
ISSN
0003-2697

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A radioenzymatic ultramicro method appli
โœ Michael N. Berry; Ronald D. Mazzachi; Ainsley H. Chalmers ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 633 KB

A radioenzymatic method suitable for the estimation of nanomole amounts of a wide range of metabolites is described. The method involves the conversion of [ l-'4C]a-ketoglutarate to [ l-'4C]glutamate by NADH or NADPH generated stoichiometrically in preliminary reactions specific for the metabolite u