An RNA-binding protein homologue that promotes sporulation-specific gene expression inSaccharomyces cerevisiae
✍ Scribed by Soushko, Maria; Mitchell, Aaron P.
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 157 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
Prior studies have shown that S. cerevisiae rim4 mutations cause reduced expression of a sporulation-speci®c reporter gene and sporulation. We report here that RIM4 (ORF YHL024W) is a putative RNA-binding protein of the RRM class that is expressed at elevated levels early in meiosis. Mutations in the two RRMs reduce or abolish sporulation and, in some cases, cause reduced Rim4p expression. RIM4 is required for expression of early and middle sporulation-speci®c genes. Unlike other meiotic regulatory genes, RIM4 is required for full gene activation through both the Ime1p and Ime2p pathways. The rim4D defect in activation by Ime2p is suppressed by a hyperactive Ime2p derivative. These observations argue that Rim4p may act upstream of Ime2p, perhaps in a nutritional sensing pathway.