An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree
✍ Scribed by M. E. Devey; T. A. Fiddler; B. H. Liu; S. J. Knapp; D. B. Neale
- Book ID
- 104670734
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 549 KB
- Volume
- 88-88
- Category
- Article
- ISSN
- 0040-5752
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✦ Synopsis
A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine.