Micro-HPLC-IP detection system in separa
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Jinno, Kiyokatsu ;Nakanishi, Shoji
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Article
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1983
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John Wiley and Sons
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English
⚖ 174 KB
Trout samples were prepared by homogenizing 10 g muscle with 50 m l O . 6 ~ perchloric acid. To 10 ml filtrate was added 10 ml0.2M KHpP04 buffer adjusted to pH 7 and containing 0.51 7~ KOH. The finalextractwhich hadthesamepH asthe bufferwas heldinicefor 1 h before filtering to remove the precipitate