An outbreak of parvovirus b19 infection; a study of clinical manifestations and the incidence of fetal loss
✍ Scribed by J. R. Kerr; H. J. O’Neill; P. V. Coyle; W. Thompson
- Book ID
- 112947147
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 371 KB
- Volume
- 163
- Category
- Article
- ISSN
- 1863-4362
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Ninety-eight samples from 80 cases of spontaneous abortions after fetal death or hydrops fetalis from 12,000 pregnant women were examined using PCR. DNA was extracted from amniotic fluid, fetal blood, ascitic fluid and fetal biopsies or placenta specimens using QIA amp kits (QIAGEN). A 270-bp length
A radioimmunoassay for human parvovirus B19 IgM was developed using virus antigen derived from infected fetal liver obtained post mortem. The specificity and sensitivity of this assay, compared with an established radioimmunoassay using serum antigen, was determined by testing 126 sera by both techn