AN ORGAN-CULTURE METHOD FOR HUMAN COLORECTAL MUCOSA USING SERUM-FREE MEDIUM

✍ Scribed by MOORGHEN, M.; CHAPMAN, M.; APPLETON, D. R.

• Book ID: 101221509
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 384 KB
• Volume: 180
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199609)180:1<102::aid-path613>3.0.co;2-s

✦ Synopsis

This study describes an explant organ-culture system in which human colonic mucosa can be maintained for prolonged periods in serum-free medium. Following an initial phase of epithelial cell loss, there was intense regenerative activity, with the reformation of tubular crypts. Estimation of crypt lengths revealed a marked reduction after 5 and 9 days in culture with corresponding increases in labelling indices for the whole crypt. The shapes of bromodeoxyuridine (BrdU)-flash-labelling distribution curves were consistent with a proliferative compartment situated within the lower two-thirds of the crypt. We conclude that this is a useful in vitvo model for the study of the effects of growth factors and growth-inhibitory agents in respect of cell proliferation in human colonic mucosa.