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An optical technique for remote focusing in microscopy

✍ Scribed by E.J. Botcherby; R. Juškaitis; M.J. Booth; T. Wilson

Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
218 KB
Volume
281
Category
Article
ISSN
0030-4018

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✦ Synopsis

We describe the theory of a new method of optical refocusing that is particularly relevant for confocal and multiphoton microscopy systems. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free refocusing can be achieved over an axial scan range of 70 lm for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method enables high axial scan speeds without mechanical interference between the objective lens and the specimen.

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