An operative approach to correct CD spectra distortions due to absorption flattening
✍ Scribed by Ettore Castiglioni; France Lebon; Giovanna Longhi; Roberto Gangemi; Sergio Abbate
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 94 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0899-0042
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✦ Synopsis
Abstract
We discuss the influence of absorption flattening (AF) on CD spectra, aiming at finding a simple way to compensate the induced distortions. A simple algebraic formalism is proposed based on an unconventional measuring approach, which makes use of a commercial unit. Validation of the proposed compensation method for AF is conducted not only on the already studied CD Vis spectral region of a Co chiral complex with the use of an AF emulator, but also on the far UV CD spectra of poly‐L‐glutamic acid suspensions with different turbidity levels. Comparison with some correction factors proposed in the literature is presented. Chirality, 2008. © 2008 Wiley‐Liss, Inc.