An on-column capillary gas chromatographic method for the analysis of synthetic pyrethroid residues on fish eggs
✍ Scribed by Bolygó, Elek ;Hadfield, Stephen T.
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 362 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
A sensitive and reproducible residue method is described for the determination of cypermethrin and deltamethrin in fish eggs. The limit of determination of 0.0005 mg kg^−1^ is achieved using only a very small sample size; typical sample weights lie in the range of 0.1‐1 g of fathead minnow or trout eggs. The method involves tissue homogenization (tube homogenizer) followed by hot acetonitrile extraction, then sequential C~8~, aluminum oxide, and silica gel clean‐up. The clean‐up is carried out in glass columns using cotton wool plugs in order to minimize potential sample contamination by plasticizers. Residues are determined by capillary chromatography using automated on‐column injection with electron‐capture detection. Confirmation of residues by gas chromatography‐mass spectrometry using selected ion monitoring (SIM), is also described.