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An NMR self-diffusion study of the interactions between spermidine and oligonucleotides

✍ Scribed by Bo Andreasson; Lars Nordenskiöld; William H. Braunlin

Publisher
Wiley (John Wiley & Sons)
Year
1998
Tongue
English
Weight
823 KB
Volume
38
Category
Article
ISSN
0006-3525

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✦ Synopsis

Spermidine and Oligonucleotides

Sc.lfd(jitsion coctfficients have been determined by pulsedfield gradient nmr methods,for spermidine in solutions qf Ihc oligonzicl~.otid~~.~ d(GC), and d(GGAA TTCC) . The selfdifliision behavior of spermidine in solution ofd(GC14 is ver-v similar to that observed previousl-v ,fbr tnrtli~ilspermidine (complctely N-methylated spermidine) . Moreover, the self diflusion hehaviors c?Jsperrnidine in solutions ofd(GC), and d(GGAA TTCC) are also quite similar, indicating that there is no signlficant influenceon on self-d(ffusion of oligonucleotide base composition.

Furthermore, seI/4$fiision coeficients efthe oligonucleotide L ~( G C ) ~

show on1.v a small dependencc) on oligonucleotide concentration, and no measurable dependmce on sodium ion or magnesium ion concentration.

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## Abstract __N__‐Methylacetamide (NMA) is known to self‐associate in solution through the formation of hydrogen bonds. This behavior, and the presence in the molecule of an amide bond, make this an interesting model for study since the oligomer can be considered to be a protein analogue. The aims