An NF-χB-like element plays an essential role in interleukin-1-mediated costimulation of the mouse interleukin-2 promoter
✍ Scribed by Kirstin Strieker; Edgar Serfling; Peter H. Krammer; Werner Falk
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 855 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Interleukin-1 (IL-1) costimulation is required for efficient IL-2 synthesis and IL-2 receptor (IG2R) expression of T cells. The molecular events leading to these effects are largely unknown. We utilized an IL-1-responsive and an IL-1non-responsive subclone of the mouse thymoma cell line EL4 to investigate how IL-1 activates IL-2 gene expression.We correlated I L 2 promoter activity with the activity of the endogenous IL-2 gene, thereby showing the biological significance of our results. Our experiments provide new functional data showing that a major target of IL-1 mediated costimulation is the xB-like site, T cell element distal TCEd (GGGATTTCAC), of the IL-2 promoter.