An isolated giant axon preparation from the lobster nerve cord. Dissection, physical structure, transsurface potentials and microinjection
✍ Scribed by Tobias, Julian M. ;Bryant, S. H.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1955
- Tongue
- English
- Weight
- 1007 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0095-9898
No coin nor oath required. For personal study only.
✦ Synopsis
Tlc.puitinent of Pli?ysiology, IinrversLty of Cliicugo ('clrtaiii espcbrirnents 011 iierve call for the use of so-called giant axoiis. Heretofore the only isolated giant axons exteiisively used have been those of the cephalopods, which, how-(TCY, a i ~ a ~a i l a b l e oiily at the wacoast and, except for a few iwlmi.atories, oiily tluriiig the summer. It recently became desii*al)le for tlie wi-itcrs to have such a preparation available tlic year around iii tlic niidwest. Tlie present report describes the isolated giaiit ax011 preparation developed to fill this need. Obtaincd from the 1ol)ster vciitral iicrvc cord it has turned out to I)c a vc.i.satilc biological tool.
Tlic 1ol)stei~ used (Hornaim arnei*icanus) have been shipped to ('hicago from tlie cast coast. They arrive packed in seaweed, iii barrels iced iii such a way as to avoid contact by the aiiirrials with fi-esli water. Obtained within a few hours after arrival, they a r e w.apped with moist seaweed in 5 or 6 layers of heavy paper aiicl are at all tirncs kept refrigerated. Riinirrial hanrlliiig aiid rriiriimal exposure to air favor survival.
' This woik Iins bceii supported in p a r t by n g r m t froin t h e Uiiited States Piiblic TTealtli Service, l)ivisioii of Neurological Disc:ises nnrl Rlindness, and i n p r t 117 n grant frow the 131.. Wallace C. m i l Clara A. Ahhott Mrmorinl Fund of the r i i i w r b i t r ( f Chicago.
Present nildrcw : Tkpartment of Plinrnxicologp, TJiiivcrsity of ('incinnati Scliool of Rfcvliciiic~.