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An Ion-Pairing High-Performance Liquid Chromatographic Method for the Direct Simultaneous Determination of Nucleotides, Deoxynucleotides, Nicotinic Coenzymes, Oxypurines, Nucleosides, and Bases in Perchloric Acid Cell Extracts

✍ Scribed by Donato Di Pierro; Barbara Tavazzi; Carlo Federico Perno; Marco Bartolini; Emanuela Balestra; Raffaele Caliò; Bruno Giardina; Giuseppe Lazzarino


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
97 KB
Volume
231
Category
Article
ISSN
0003-2697

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✦ Synopsis


be involved in energy metabolism and in DNA and RNA synthesis, resulting therefore of great advantage in An ion-pairing high-performance liquid chromatographic method for the direct and simultaneous deter-cell biology studies. ᭧ 1995 Academic Press, Inc. mination of nucleotides, deoxynucleotides, cAMP, nicotinic coenzymes, oxypurines, nucleosides, and bases in perchloric acid cell extracts is presented. By using an Alltima C-18, 250 1 4.6-mm, 5-mm particle size col-1 This work was partly supported by a CNR Grant ''P.F.: Invecchiadetermination of concentrations either of other deoxymento. '' 2 To whom correspondence should be addressed.

nucleotides or of other metabolic intermediates such as 407