A synthetic gene for the human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in HEK293 cells with the bioluminescent Ca 2Ψ indicator protein aequorin. The dosedependent response of the receptor to motilin was demonstrated using transient transfections, a
An investigation of the ionic calcium assay based on the jellyfish protein aequorin
β Scribed by K.T. Izutsu; S.P. Felton; I.A. Siegel; J.I. Nicholls; J. Crawford; J. McGough; W.T. Yoda
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 899 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
The ionic calcium assay based on the luminescence of the jellyfish protein aequorin was studied.
A rapid mixing device was found to be necessary for good reproducibility.
The accuracy of the procedure described could depend on the association constants of the calcium chelating agents involved in the test solutions.
π SIMILAR VOLUMES
## Abstract Novel proteinβbased nanocomposites were well prepared by __in vivo__ synthesis and coβprecipitation of soy protein isolate (SPI) with calcium carbonate (CaCO~3~) in an aqueous solution. The resultant CaCO~3~ in the nanocomposites was identified as calciteβ and aragoniteβtype, respective