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An internal standard for porphyrin analysis

✍ Scribed by Robert E. Carlson; Ramani Sivasothy; David Dolphin; Melvyn Bernstein; Ally Shivji

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 404 KB
Volume: 140
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90178-7

No coin nor oath required. For personal study only.

✦ Synopsis

The routine clinical analysis of the porphyrin precursors of heme requires an internal standard to determine the efficiency of the analytical procedure used. 2-Vinyl-4-hydroxymethyldeuteroporphyrin IX has been prepared as an internal standard. Its application to porphyrin analysis has been demonstrated using high-performance liquid chromatographic resolution of the uroto protoporphyrins in normal and potphyric urine.

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