An integrated comparative map of the porcine X chromosome

✍ Scribed by S. A. McCoard; S. C. Fahrenkrug; L. J. Alexander; B. A. Freking; G. A. Rohrer; T. H. Wise; J. J. Ford

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 976 KB
• Volume: 33
• Category: Article
• ISSN: 0268-9146
• DOI: 10.1046/j.1365-2052.2002.00878.x

✦ Synopsis

The objectives of this study were to assign both microsatellite and gene‐based markers on porcine chromosome X to two radiation hybrid (RH) panels and to develop a more extensive integrated map of SSC‐X. Thirty‐five microsatellite and 20 gene‐based markers were assigned to T43RH, and 16 previously unreported microsatellite and 15 gene‐based markers were added to IMpRH map. Of these, 30 microsatellite and 12 gene‐based markers were common to both RH maps. Twenty‐two gene‐based markers were submitted to BLASTN analysis for identification of orthologues of genes on HSA‐X. Single nucleotide polymorphisms (SNPs) were detected for 12 gene‐based markers, and nine of these were placed on the genetic map. A total of 92 known loci are present on at least one porcine chromosome X map. Thirty‐seven loci are present on all three maps; 31 loci are found on only one map. Location of 33 gene‐based markers on the comprehensive map translates into an integrated comparative map that supports conservation of gene order between SSC‐X and HSA‐X. This integrated map will be valuable for selection of candidate genes for porcine quantitative trait loci (QTLs) that map to SSC‐X.