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An instance of temperature-dependent elution order of enantiomers from a chiral brush-type HPLC column

✍ Scribed by Pirkle, William H. ;Murray, Patrick G.

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 349 KB
Volume: 16
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240160505

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✦ Synopsis

Abstract

Typically, reduction of column temperature increases the enantioselectivity of a chiral stationary phase. An instance in which progressive reduction of temperature initially reduces enantioselectivity, then inverts the elution order of the enantiomers, and finally causes enantioselectivity to increase has been observed. This behavior is related to the nature and concentration of the polar modifier in the mobile phase, and requires particular chiral phase‐analyte‐mobile phase combinations. A rationalization is presented as to the possible origin of this behavior. This rationale may aid in finding other examples of this temperature‐dependent elution order of enantiomers and ultimately increase our understanding of chiral recognition processes.

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