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An indicator displacement assay with independent dual wavelength emission

✍ Scribed by Shaohui Zhang; Timothy E. Glass


Publisher
Elsevier Science
Year
2010
Tongue
French
Weight
313 KB
Volume
51
Category
Article
ISSN
0040-4039

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✦ Synopsis


A multifunctional metallo-receptor was designed with both metal and boronic acid binding groups. A sensor ensemble was prepared using the metallo-receptor and the fluorescent dye ARS. The dye produced two distinct fluorescent bands from interaction with the boronic acid and the metal respectively. Partial displacement of the dye by simple analytes led to different fluorescent signatures than full displacement. This differential response provided easy discrimination of the individual analytes.