An increase of intracellular free Ca2+ is essential for spontaneous meiotic resumption by mouse oocytes
✍ Scribed by De Felici, Massimo ;Dolci, Susanna ;Siracusa, Gregorio
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 499 KB
- Volume
- 260
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
The involvement of calcium ions in the mechanism of meiotic resumption has been studied in mouse oocytes made resistant to the lethal effects of calcium‐free medium (CFM) by zona pellucida removal (De Felici et al., '89). We show here that such oocytes undergo meiotic resumption in CFM (as evaluated by germinal vesicle breakdown, GVBD) at a rate comparable to that shown by oocytes cultured in medium containing 1.7 m__M__ Ca^2+^. The addition to CFM of 50 u__M__ Quin2/AM (a membrane permeable, high affinity Ca^2+^ chelator) totally prevents GVBD, while purported antagonists of Ca^2+^ release from intracellular stores, such as 150 u__M__ 8‐(N, N‐diethylamino)octyl‐3‐4‐5 trimethoxybenzoate (TMB‐8) or 300 u__M__ chlortetracycline, only cause a slight meiotic delay. On the other hand, if the oocytes are pre‐incubated for 30 min in CFM supplemented with 100 u__M__ TBM‐8 plus 0.2 m__M__ dibutyryl‐cyclic AMP (dbcAMP, a reversible inhibitor of GVBD), and then cultured in the same medium, without dbcAMP, a sustained inhibition of meiotic maturation is obtained.
Our observations suggest that an increase in intracellular free Ca^2+^ is essential for meiotic resumption by mouse oocytes; in the experimental absence of external Ca^2+^, release of the cation from internal stores is sufficient to allow meiotic resumption.