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An increase in the transglycosylation activity of Saccharomycopsis α-amylase altered by site-directed mutagenesis

✍ Scribed by Ikuo Matsui; Kazuhiko Ishikawa; Sachio Miyairi; Sakuzo Fukui; Koichi Honda


Book ID
113268635
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
295 KB
Volume
1077
Category
Article
ISSN
0167-4838

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Increasing the transglycosylation activi
✍ Sandra W.A. Hinz; Chantal H.L. Doeswijk-Voragen; Roelof Schipperus; Lambertus A. 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 544 KB

## Abstract The α‐galactosidase (AGA) from __Bifidobacterium adolescentis__ DSM 20083 has a high transglycosylation activity. The optimal conditions for this activity are pH 8, and 37°C. At high melibiose concentration (600 mM), approximately 64% of the enzyme–substrate encounters resulted in trans