𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An in vivo study of tricalcium phosphate and glutaraldehyde crosslinking gelatin conduits in peripheral nerve repair

✍ Scribed by Ming-Hong Chen; Pei-Ru Chen; Mei-Hsiu Chen; Sung-Tsang Hsieh; Jing-Shan Huang; Feng-Huei Lin

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
728 KB
Volume
77B
Category
Article
ISSN
1552-4973

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In order to modulate the mechanical properties of gelatin, we previously developed a biodegradable composite composed by tricalcium phosphate and glutaraldehyde crosslinking gelatin (GTG) feasible for surgical manipulation. In this study, we evaluated the in vivo applications of GTG conduit for peripheral nerve repair. The effect of sciatic nerve reconstruction was compared between resorbable permeable GTG conduits and durable impermeable silicone tubes. Traditional methods of assessing nerve recovery following peripheral nerve repair including histomorphometric and electrophysiologic features were conducted in our study. In addition, autotomy score and sciatic function index (SFI) in walking tract analysis were used as additional parameters for assessing the return of nerve function. Twenty‐four weeks after sciatic nerve repair, the GTG conduits were harvested. Microscopically, regeneration of nerves was observed in the cross‐section at the mid portion of all implanted GTG conduits. The cross‐sectional area of regenerated nerve of the GTG group was significant larger than that of the silicone group. In the compound muscle action potentials (CMAP), the mean recovery index of CMAP amplitude was 0.24 ± 0.02 for the silicone group, 0.41 ± 0.07 for the GTG group. The mean SFI increased with time in the GTG group during the evaluation period until 24 weeks. Walking tract analysis showed a higher SFI score in the GTG group at both 12 and 24 weeks. The difference reached a significant level at 24 weeks. Thus, the histomorphometric, electrophysiologic, and functional assessments demonstrate that GTG can be a candidate for peripheral nerve repair. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006

📜 SIMILAR VOLUMES

Fabrication and evaluation of a biodegra
Fabrication and evaluation of a biodegradable proanthocyanidin-crosslinked gelatin conduit in peripheral nerve repair
✍ Bai-Shuan Liu 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 624 KB

## Abstract This study proposed a novel and biodegradable nerve guide conduit in its applicability to peripheral nerve regeneration. A naturally occurring proanthocyanidin (PA) was selected as a cross‐linking reagent in preparing the PA‐crosslinked gelatin (PCG) conduit. Experimental results indica

Gelatin–tricalcium phosphate membranes i
Gelatin–tricalcium phosphate membranes immobilized with NGF, BDNF, or IGF-1 for peripheral nerve repair: An in vitro and in vivo study
✍ Ming-Hong Chen; Pei-Ru Chen; Mei-Hsiu Chen; Sung-Tsang Hsieh; Feng-Huei Lin 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 744 KB

## Abstract In the present study, NGF, BNDF from the neurotrophin family and IGF‐1 were covalently immobilized on gelatin–tricalcium phosphate (GTG) membrane using carbodiimide. We investigated the effects of these growth factors released from the GTG composites on cultured PC12 cells and sciatic n

Effects of nerve growth factor from geni
Effects of nerve growth factor from genipin-crosslinked gelatin in polycaprolactone conduit on peripheral nerve regeneration—In vitro and in vivo
✍ Chen-Jung Chang 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 568 KB

## Abstract The gelatin solution crosslinked by genipin (0, 0.1, 0.5, 1.0, and 1.5% w/w) was studied as a nerve growth factor (NGF) carrier (GGp0, GGp0.1, GGp0.5, GGp1.0, and GGp1.5) in a polycaprolactone conduit in large‐gap nerve regeneration. The GGp0 and GGp0.1 displayed the highest activity of

Laser-activated solid protein bands for
Laser-activated solid protein bands for peripheral nerve repair: An in vivo study
✍ Lauto, Antonio; Trickett, Rodney; Malik, Richard; Dawes, Judith M.; Owen, Earl R 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 314 KB 👁 2 views

## Background and objective: Severed tibial nerves in rats were repaired using a novel technique, utilizing a semiconductor diode-laser-activated protein solder applied longitudinally across the join. welding was produced by selective laser denaturation of solid solder bands containing the dye indo

Effects of unidirectional permeability i
Effects of unidirectional permeability in asymmetric poly(DL-lactic acid-co-glycolic acid) conduits on peripheral nerve regeneration: An in vitro and in vivo study
✍ Chen-Jung Chang; Shan-hui Hsu; Hung-Jen Yen; Han Chang; Shih-Kuang Hsu 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 723 KB

## Abstract The high outflow permeability of the nerve conduit used to emit the drained waste generated from the traumatized host nerve stump is critical in peripheral nerve regeneration. Our earlier studies have established that asymmetric conduits fulfill the basic requirements for use as nerve g