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An in vitro model system for cytoskeletal confinement

✍ Scribed by Köster, Sarah ;Pfohl, Thomas

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
232 KB
Volume
66
Category
Article
ISSN
0886-1544

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✦ Synopsis

Abstract

The motility, shape, and functionality of the cell depend sensitively on cytoskeletal mechanics which in turn is governed by the properties of filamentous proteins ‐ mainly actin, microtubules, and intermediate filaments. These biopolymers are confined in the dense cytoplasm and therefore experience strong geometric constraints on their equilibrium thermal fluctuations. To obtain a better understanding of the influence of confinement on cytoskeletal filaments we study the thermal fluctuations of individual actin filaments in a microfluidic in vitro system by fluorescence microscopy and determine the persistence length of the filaments by analyzing the radial distribution function. A unique feature of this method is that we obtain the persistence length without detailed knowledge of the complete contour of the filament which makes the technique applicable to a broad range of biological polymers, including those with a persistence length smaller than the optical resolution. Cell Motil. Cytoskeleton 2009. © 2009 Wiley‐Liss, Inc.

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