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An in situ hybridization screen for the rapid isolation of differentially expressed genes

✍ Scribed by T. Henrich; J. Wittbrodt

Publisher
Springer-Verlag
Year
2000
Tongue
English
Weight
546 KB
Volume
210
Category
Article
ISSN
0949-944X

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## Abstract An in situ hybridization expression screen using a signal sequence trap system has been conducted in zebrafish to isolate cDNAs that encode secreted proteins. Random clones (secreted expressed sequence tags; sESTs) were sequenced from zebrafish embryonic (18–24 hr postfertilization) and