An improvement of the Coomassie Blue dye binding method allowing an equal sensitivity to various proteins: application to cerebrospinal fluid

Application of an improved biuret method

Application of an improved biuret method to the determination of total protein in urine and cerebrospinal fluid without concentration step by use of Hitachi 7170 auto-analyzer

✍ Xu Guobing; Jiao Lili; Zhu Lihua; Xia Tiean 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 47 KB

## Abstract A biuret automated colorimetric assay for total protein in urine and cerebrospinal fluids was established. The procedures were as follows. Acidify all urine sample before analysis. Add precipitant Na~2~WO~4~ to urine samples. After 10 min, centrifuge, decant the supernatant fluid, drain