An improvement in the hummel chymotrypsin assay

✍ Scribed by Suk-Hee Kang; Morton S. Fuchs

• Book ID: 102628281
• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 187 KB
• Volume: 54
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(73)90270-4

✦ Synopsis

A well-known and often-used assay for the determination of esterolytic activity of chymotrypsin is the Hummel method (1). It is based on the rate of hydrolysis of N-benzoyl-n-tyrosine ethyl est'er (BTEE) as determined by the change in absorbancy at 256 nm. The assay is the first choice of many laboratories because it is relatively inexpensive, is easily and rapidly performed and also because BTEE is resistant to tryptic hydrolysis (1). We have found that by simply adjusting the concentrations of methanol and the substrate (BTEE) in the reaction mixture the sensitivity of this assay can be increased more than two-fold.