An improved protocol for the preparation
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Thompson, J. R.; Register, E.; Curotto, J.; Kurtz, M.; Kelly, R.
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Article
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1998
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John Wiley and Sons
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English
⚖ 98 KB
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Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f