✦ LIBER ✦
An improved technique for the preparative electrophoresis and electroelution of high molecular weight ribosomal RNA
✍ Scribed by Rose Ann Cattolico; Raymond F. Jones
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 648 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A new technique for casting flat 0.5% agarose-2.4% acrylamide preparative gels is described in this communication. The casting method presented completely eliminates mechanical problems and technical complications often encountered in producing the homogeneous loading platform critical to the entrance and proper migration of RNA species on low pore composite gels. The gels formed can be used to separate milligram quantities of RNA with excellent resolution and a method for the electroelution of a specific RNA species is also presented.