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An Improved System for Ribonuclease Ba Expression

โœ Scribed by A.L. Okorokov; R.W. Hartley; K.I. Panov


Book ID
115644860
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
440 KB
Volume
5
Category
Article
ISSN
1046-5928

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The eukaryotic serine protease, rat anionic trypsin, and various mutants created by site-directed mutagenesis have been heterologously expressed in Escherichiu coZi. The bacterial alkaline phosphatase (phoA) promoter was used to control the expression of the enzymes in an induced or constitutive fas