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An improved SUMO fusion protein system for effective production of native proteins

✍ Scribed by Chien-Der Lee; Hui-Chien Sun; Su-Ming Hu; Ching-Feng Chiu; Atthachai Homhuan; Shu-Mei Liang; Chih-Hsiang Leng; Ting-Fang Wang


Book ID
111752289
Publisher
Cold Spring Harbor Laboratory Press
Year
2008
Tongue
English
Weight
412 KB
Volume
17
Category
Article
ISSN
0961-8368

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Linkers for improved cleavage of fusion
✍ Samantha Lien; Steven J. Milner; Lloyd D. Graham; John C. Wallace; Geoffrey L. F πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 117 KB

## Abstract Addition of an N‐terminal fusion partner can greatly aid the expression and purification of a recombinant protein in __Escherichia coli__. We investigated two genetically engineered proteases designed to remove the fusion partner after the protein of interest has been expressed. Recombi