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An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation

✍ Scribed by Ming Sun; Seymour Zigman

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
453 KB
Volume
90
Category
Article
ISSN
0003-2697

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✦ Synopsis

Superoxide dismutase activity was assayed in terms of its ability to inhibit the radical-mediated chain-propagating autoxidation of epinephrine. The enzyme assay based on adrenochrome absorption at 480 nm has been improved by measuring the absorption change at 320 nm. This alternative procedure was found to be 6 to 10 times more sensitive and more consistent than that measured at 480 nm.

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