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An improved Re/W separation protocol for preparation of carrier-free186Re

✍ Scribed by Z. H. Zhu; X. Y. Wang; Y. H. Wu; Y. F. Liu


Publisher
Springer
Year
1997
Tongue
English
Weight
241 KB
Volume
221
Category
Article
ISSN
1588-2780

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An improved protocol for the preparation
✍ Thompson, J. R.; Register, E.; Curotto, J.; Kurtz, M.; Kelly, R. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 98 KB πŸ‘ 2 views

Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f