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An improved radioimmunoassay method for the detection of IgG antibodies against cytomegalovirus

โœ Scribed by N.J. Berry; J.E. Grundy; P.D. Griffiths


Book ID
119114383
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
544 KB
Volume
13
Category
Article
ISSN
0166-0934

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Two prokaryotically expressed fusion proteins encompassing amino acids 484-650 (AD-I) and 27-100 (AD-2) of glycoprotein gp58/116 of human cytomegalovirus (HCMV) were purified from E. coli lysates and used in ELISA to determine antibody levels in human sera. The specificity of the test was establishe