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An improved procedure for the simultaneous purification in high yield of peroxisomes, lysosomes, and mitochondria from rat liver

โœ Scribed by G. B. Sephton; K. F. Buechler; J. M. Lowenstein


Book ID
112522424
Publisher
Springer Vienna
Year
1990
Tongue
English
Weight
778 KB
Volume
153
Category
Article
ISSN
0033-183X

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An improved procedure for the isolation of mitochondria in high yields from normal and oxygen-deficient myocardium is described. The heart muscle is digested with Nagarse and homogenized simultaneously using a Polytron tissue homogenizer. Mitochondria are isolated by differential centrifugation, and