An improved and simplified procedure for enzymatic digestion of proteins bound to polyvinylidene difluoride (PVDF) membranes for obtaining internal protein sequence data is presented. This improved procedure is compatible with various enzymes (trypsin, endoproteinase Lys-C, endoproteinase Glu-C, and
โฆ LIBER โฆ
An improved procedure for the enzymatic analysis of prostaglandins in the nanogram range
โ Scribed by Franz M. Matschinsky; Dennis Shanahan; Jeanette Ellerman
- Book ID
- 102984165
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 700 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
Prostaglandin dehydrogenase is purified from beef lung and optimal storage conditions are established for the enzyme. Improved assay conditions are worked out for the stoichiometric enzymatic analysis of prostaglandins in the nanogram range. The method appears to be applicable to all naturally occurring prostaglandins.
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