An improved microalbumin method (µALB_2) with extended analytical measurement range evaluated on the ADVIA® chemistry systems
✍ Scribed by Pradip Datta; Amitava Dasgupta
- Book ID
- 102311639
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 432 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (mALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We evaluated analytical performances of this new method. All ADVIA Chemistry Systems use the same microalbumin reagent packs, mALB_2 calibrators, and commercial controls. The within-run and total CVs of the improved method with two-level BioRad Liquichek Urine Chemistry controls ($2 and 9 mg/dl ALB) and a urine pool ($29 mg/dl ALB) on all ADVIA Chemistry systems were o4.1 and o6.1%, respectively (40 replicates per sample). The analytical range/linearity of the method (all ADVIA systems) was from 0.3 mg/dl to the ALB concentration in the highest level of calibrator ($38-42 mg/dl). The improved method (mALB_2) on the ADVIA 1650/ 1800 (y) correlated well with both the Beckman DXC 800 Microalbumin and the old microalbumin method on the ADVIA 1650/1800 analyzers. The improved method showed o10% interference with 16 chemicals from acetaminophen to uric acid that may be present in urine. The improved method has a minimum of 60 days' onsystem stability on all systems with the calibration frequencies of (with/without a Reagent Container insert) 20/30 days (ADVIA1200), 50/60 days (ADVIA1650/ 1800), and 20/60 days (ADVIA2400). No prozone was observed with the method on any platform up to the highest ALB concentration tested in a sample (4,000 mg/dl).