Most peroxisomal disorders can be detected via analysis of very long chain fatty acids (VLCFA) and phytanic acid in plasma or serum. Previous methods utilizing gas-liquid chromatography (GLC) alone are time consuming and are hampered by interference from cholesterol derivatives. We describe here a G
✦ LIBER ✦
An improved method of characterizing fatty acids by equivalent chain length values
✍ Scribed by Stránský, Karel ;Jursík, Tomáš ;Vítek, Antonín ;Skořepa, Jiří
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 980 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
A method has been developed for calculating more accurate and reproducible equivalent chain length (ECL) values of 33 straight‐chain fatty acids (FA), with various positions and numbers of cis double bonds, chromatographed on DB‐Wax and DB‐1 columns. The dependence of ECL values on the position and the number of double bonds is described, as is a method of utilizing these relationships for the characterization of FA by gas chromatography.
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