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An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays

✍ Scribed by Inca Ghosh; Luo Sun; Thomas C. Evans Jr.; Ming-Qun Xu


Book ID
113716851
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
341 KB
Volume
293
Category
Article
ISSN
0022-1759

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We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld