An improved method for the study of equine haptoglobin heterogeneity

✍ Scribed by E. M. Milne

• Publisher: Springer Netherlands
• Year: 1990
• Tongue: English
• Weight: 485 KB
• Volume: 14
• Category: Article
• ISSN: 0165-7380
• DOI: 10.1007/bf00367054

✦ Synopsis

Mime, EM., 1990. An improved method for the study of equine haptoglobin heterogeneity. Veterimny Research Communicalions, 14, (6), 433-439 Equine serum haptoglobin was separated by polyacrylamide gel iscelectric focusing and visualized by protein staining or Western blotting. Conventional protein staining revealed up to three bands in the p1 range 4.17 to 4.44. The blotting technique, however, showed an anodal group of 8 to 10 bands with a p1 range of 4.11 to 452 and a cathodal group of 4 to 6 bands with a range of 4.55 to 5.14. The blotting method revealed that equine haptoglobin migrates outside the prealbumin area, in contrast to previous reports.