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An improved method for the colorimetric assay of lipase activity using an optically clear medium

✍ Scribed by G. Renard; J. Grimaud; A. El Zanf; M. Pina; J. Gralle

Book ID
112775486
Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
287 KB
Volume
22
Category
Article
ISSN
0024-4201

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We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld