An improved method for preparing cell suspensions used for the cloning of human tumors
โ Scribed by J. Gioanni; C.M. Lalanne
- Book ID
- 115824014
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 223 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0009-8981
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An enzymatic method is described for disaggregation of viable tumor cells from human solid tumors. The enzymatic cocktail consists of 0.1 % collagenase, 0.01 % hyaluronidase, and 0.002 % deoxyribonuclease. After mechanical mincing of the tumor tissue, tumor specimens are dissociated by incubation in
Cell viability or cell death is an important variable to monitor in many studies of host/pathogen interactions. However for studies that focus on events within the first few hours of the interaction, many of the viability assays currently being used are either too laborious and time consuming or mea