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An Improved Method for Polymerase Chain Reaction Using Whole Yeast Cells

✍ Scribed by Hong Wang; Susanne E. Kohalmi; Adrian J. Cutler


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
87 KB
Volume
237
Category
Article
ISSN
0003-2697

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Analysis of the products of the RNA-directed DNA polymerase reaction using glycerol-substituted polyacrylamide gels has proven superior to the glycerol gradient method for quantitative assay of a series of nucleic acid samples. To overcome the phenomenon of a smearing of background radioactive count