✦ LIBER ✦
An improved method for destroying mouse blastomeres electrically inside the zona pellucida and the in vitro development of the surviving blastomeres
✍ Scribed by Tojo, Hideaki ;Ogita, Zen-Ichi
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 583 KB
- Volume
- 229
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
An improved method for isolating mouse blastomeres by electrically destroying the other blastomeres inside the zona pellucida is described. Instruments required are two micromanipulators connected to a n inverted microscope and an electronic stimulator equipped with an isolator. The target blastomere in which a glass microelectrode is inserted disintegrates completely within a few seconds after a direct current is applied. Complete destruction of a blastomere requires more than 60 FA delivered at 8 V. Destruction of