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An improved method for analysis of technetium radiolabeled antibody by size exclusion HPLC

✍ Scribed by S.N. Muddukrishna; P. Jorge; W. Machner; T.R. Sykes; A.A. Noujaim

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
979 KB
Volume
45
Category
Article
ISSN
0969-8043

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✦ Synopsis

A quantitative

radio HPLC method for the analysis of radiolabeled YY'"T~ compounds, in particular labeled antibodies or proteins, is described. The method is based on the quantitative re-oxidation of the reduced column absorbed 9YmT~ species to pertechnetate using hydrogen peroxide. The pertechnetate is quantitatively eluted from the column. The method can be used as a stand alone technique for determining the true radiolabeling yield of labeled proteins/antibodies. The method has been automated for routine use and the typical analysis time is 30min.

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