An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils

✍ Scribed by Trevor T. Hansel; I.Jolanda M. De Vries; Thomas Iff; Sylvia Rihs; Maciej Wandzilak; Susanne Betz; Kurt Blaser; Christoph Walker

• Book ID: 118902403
• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 453 KB
• Volume: 145
• Category: Article
• ISSN: 0022-1759
• DOI: 10.1016/0022-1759(91)90315-7

✦ Synopsis

A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenylalanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils.