An improved histochemical method for measuring nitric oxide synthase activity
✍ Scribed by Jamshid Alaghband-Zadeh; Siroos Mehdizadeh; Anne O'Farrell; Judith Weisz; Lucille Bitensky; Joseph Chayen
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 91 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0263-6484
No coin nor oath required. For personal study only.
✦ Synopsis
The previous quantitative histochemical method for measuring nitric oxide synthase (NOS) activity in tissue sections involved the loss of about 15 per cent of the NOS, presumably from the section into the reaction medium. Two changes are now described. The ®rst is concerned with the preparation in the laboratory of the active reagent, lead ammonium citrate/acetate (LACA). The second change involves an improvement of the procedure for measuring NOS activity. The new method appears to retain all the measurable NOS activity inside the section.