An improved extraction procedure for the rapid, quantitative high-performance liquid chromatographic estimation of the main eleutherosides (b and e) in Eleutherococcus senticosus (eleuthero)
✍ Scribed by Patrick N. Yat; John T. Arnason; Dennis V. C. Awang
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 65 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0958-0344
No coin nor oath required. For personal study only.
✦ Synopsis
Eleutherosides were extracted with aqueous methanol and analysed by reversed-phase high performance liquid chromatography on a C-18 column. Recovery was better than 80% for eleutheroside B within the concentration range 500 to 10,000 mg, and similarly for eleutherosides E in the range 500 to 2,500 mg. Lower recovery values were obtained at higher concentrations for eleutheroside E due to its insolubility in alcoholic solutions. The use of trifluoroacetic acid solution in methanol was found to resolve the solubility problem. The concentration ranges over which a linearity of response for both eleutherosides could be validated were improved significantly by this adjustment.