An improved data analysis method for interleukin 2 microassay

✍ Scribed by Carol J. Burger; Klaus D. Elgert; John J. Tyson; Jeffery B. Birch

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 973 KB
• Volume: 16
• Category: Article
• ISSN: 0010-4825
• DOI: 10.1016/0010-4825(86)90005-3

✦ Synopsis

Development of the interleukin 2 (IL 2) microassay, coupled with the use of highly purified or recombinant factors has allowed a detailed examination of the mechanism of action of this important biological response modifier. However, probit analysis of the microassay data does not allow inherent error of the system to be approximated nor can units of activity be assessed for significance. A computer program was developed to analyze the validity ofeach regression line and to generate 95% confidence intervals around each line. This program employs analysis of variance, linear regression analysis and the parallel line assay to fix confidence intervals for each IL 2 unit value. The use of recombinant IL 2 as an immunomodulator in clinical settings warrants a more precise statistical method to evaluate normal fluctuations of this factor than currently in use. The development of such a method is presented here.