An improved colorimetric assay for polyols

✍ Scribed by Song Hae Bok; Arnold L. Demain

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 167 KB
• Volume: 81
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90593-0

✦ Synopsis

A new assay for polyols was developed that minimizes interference by sugars. It is based on oxidation of alditols to formaldehyde by acidic periodate, reduction of the excess periodate with L-rhamnose. and a short-time determination of the formaldehyde with the Nash reagent. When glycerol was added to various crude biological materials and was assayed, recovery was 90-106%.