๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

An improved chloramphenicol acetyltransferase assay for Plasmodium falciparum transfection

โœ Scribed by Stuart J. Lucas; Anthony A. Holder


Book ID
113802551
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
362 KB
Volume
136
Category
Article
ISSN
0166-6851

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


A highly sensitive, mixed-phase assay fo
โœ David A. Nielsen; Tsu-Chung Chang; David J. Shapiro ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 867 KB

We describe a simple, rapid yet extremely sensitive assay for chloramphenicol acetyltransferase (CAT) activity in extracts from transfected eukaryotic cells. Using our modified reaction conditions and the mixed-phase assay, less than 0.000010 unit of CAT activity in transfected cells can be reliably

An Improvement in the Pyruvate Dehydroge
โœ S.P.J. Brooks; K.B. Storey ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 411 KB

A new method for purifying arylamine acetyltransferase (AAT) has been devised using polyethylene glycol fractionation and hydroxylapatite chromatography. The new procedure gives a final yield of approximately \(70 \%\) based on activity in crude homogenates and can be performed in a single day. This